Zogulitsa

Tsatanetsatane wa Zamalonda:

Mayeso a Innovita® 2019-nCoV IgM/IgG adapangidwa kuti azitha kuzindikira pang'ono kuchuluka kwa ma antibodies ku novel coronavirus (2019-nCoV) mu seramu yamunthu, plasma kapena zitsanzo zamagazi athunthu.
2019-nCoV imaphatikizapo mapuloteni anayi akuluakulu: S protein, E protein, M protein ndi N protein.Dera la RBD la S protein limatha kumangirira ku cell cell receptor ACE2.Neutralizing antibody imatanthawuza kuthekera komanga ndi tizilombo toyambitsa matenda, ndiyeno kutsekereza tizilombo toyambitsa matenda kuti tilowe m'thupi kuti tiyambitsa matenda.Kuzindikira kwa ma antibody oletsa kuphatikizika kungagwiritsidwe ntchito kuwunika momwe ma virus alili.

Mfundo Yofunika:

Chidachi ndi mpikisano wa colloid golide wa immunochromatography kuti azindikire ma antibodies ku 2019-nCoV mu seramu yamunthu, plasma kapena zitsanzo zamagazi athunthu.Chitsanzocho chikagwiritsidwa ntchito pa chitsanzocho bwino, ngati ma antibodies a neutralizing alipo mu chitsanzo, ma antibodies a neutralizing adzachitapo kanthu ndi golidi wa colloidal otchedwa RBD antigen kuti apange chitetezo cha mthupi, ndipo malo osasunthika otchedwa RBD antigen adzatsekedwa.Kenako chitetezo chamthupi komanso chotchedwa RBD antigen popanda kumangiriza antibody chimasuntha motsatira nembanemba ya nitrocellulose.Akafika kumalo oyesera (mzere wa T), antigen yolembedwa kuti RBD antigen osamangiriza ma antibodies amakhudzidwa ndi antigen ya ACE2 yomwe idakutidwa pa nembanemba ya nitrocellulose ndikupanga mzere wofiyira.Pamene chiwerengero cha ma antibodies a neutralizing ndi apamwamba kuposa malire otsika kwambiri, mzere wofiirira-wofiira ndi wopepuka kuposa mzere wowongolera (C line) kapena palibe mzere wofiirira-wofiira wopangidwa, zotsatira zake zimakhala zabwino.Pamene chiwerengero cha ma antibodies a neutralizing ndi otsika kuposa malire otsika kwambiri odziŵika kapena palibe ma antibodies osakanikirana mu chitsanzo, mzere wofiirira-wofiira ndi wakuda kuposa mzere wolamulira, zotsatira zake zimakhala zoipa.
Mosasamala kanthu kuti chitsanzocho chili ndi ma antibodies a 2019-nCoV, pamene antibody ya IgY yolembedwa ndi golidi ya golide ikasamukira ku mzere wowongolera (C line), imagwidwa ndi mbuzi yolimbana ndi nkhuku IgY antibody yomwe ili pamzere wowongolera (C). mzere), mzere wofiirira-wofiira umapangidwa.Mzere wowongolera (C mzere) umagwiritsidwa ntchito ngati njira yowongolera.Mizere yowongolera iyenera kuwonekera nthawi zonse pazotsatira mazenera ngati njira yoyeserera ichitidwa moyenera ndipo ma reagents akugwira ntchito momwe amafunira.

Zolemba:

Njira Yoyesera:

1. Tsegulani thumba lazojambula za aluminiyamu ndikutulutsa kaseti yoyesera.
2. Ikani 40μL ya chitsanzo cha seramu/plasma kapena 60μL yamagazi athunthu pachitsanzocho.
3. Ikani 40μL (madontho 2) osungunula pachitsimecho.
4. Ikani kutentha (15 ℃ ~ 30 ℃) kwa mphindi 15-20, ndikuwerenga zotsatira.

Kutanthauzira kwa Zotsatira:

1. Zabwino: Pamene mtundu wa T uli wopepuka kuposa wa C kapena ngati palibe mzere wa T, umasonyeza zabwino zochepetsera ma antibodies.
2. Zoipa: Pamene mtundu wa mzere wa T uli wakuda kuposa kapena wofanana ndi wa C mzere, umasonyeza kuti palibe ma antibodies.
3. Zosavomerezeka: Pamene mzere wa C ukulephera kuwonekera, mosasamala kanthu kuti mzere wa T ukuwoneka kapena ayi, mayesowo ndi osavomerezeka.Bwerezani mayesowo ndi mayeso atsopano.