2019-nCoV Neutralizing Antibody Test (QDIC)
Tsatanetsatane wa Zamalonda:
Mayeso a Innovita® 2019-nCoV IgM/IgG adapangidwa kuti azindikire kuchuluka kwa ma antibody omwe amatulutsa kachilombo ka corona (2019-nCoV) mu seramu yamunthu, plasma kapena magazi athunthu (magazi a chala kapena magazi athunthu).
2019-nCoV imaphatikizapo mapuloteni anayi akuluakulu: S protein, E protein, M protein ndi N protein.Dera la RBD la S protein limatha kumangirira ku cell cell receptor ACE2.Kafukufuku wasonyeza kuti zitsanzo za anthu omwe achira matenda atsopano a coronavirus ndi zabwino zochepetsera ma antibody.Kuzindikira kwa ma antibody oletsa kuphatikizika kungagwiritsidwe ntchito kuwunika momwe ma virus amayendera komanso kuwunika pambuyo pa katemera.
Mfundo Yofunika:
Chidachi ndi kuyesa kwa quantum dot immunofluorescence chromatography assay kuti muwone ma 2019-nCoV RBD enieni a IgG osasokoneza ma antibodies mu seramu yamunthu, plasma kapena magazi athunthu (magazi a chala ndi venous magazi athunthu).Pambuyo pake chitsanzocho chikugwiritsidwa ntchito pa chitsanzo chabwino, ngati chiwerengero cha ma antibodies a neutralizing ndi apamwamba kuposa malire otsika kwambiri, ma antibodies a RBD enieni a IgG adzachita ndi gawo kapena RBD antigen yolembedwa ndi quantum dot microspheres kuti apange chitetezo cha mthupi.Kenako chitetezo chamthupi chimasuntha motsatira nembanemba ya nitrocellulose.Akafika kumalo oyesera (T mzere), pawiri adzachitapo kanthu ndi mbewa anti-anthu IgG (γ unyolo) TACHIMATA pa nitrocellulose nembanemba ndi kupanga fulorosenti mzere.Werengani mtengo wa siginecha ya fluorescence ndi chowunikira cha fluorescence immunoassay.Mtengo wa siginecha umagwirizana ndi zomwe zili ndi ma antibodies omwe ali pachitsanzo.
Kaya chitsanzocho chili ndi ma antibodies enieni a RBD kapena ayi, mzere wowongolera uyenera kuwonekera pazenera lazotsatira ngati njira yoyeserera ichitidwa bwino ndipo reagent ikugwira ntchito monga momwe idafunira.Pamene anti-Chicken IgY antibody yolembedwa ndi ma quantum dot microspheres isamukira ku mzere wowongolera (C line), imagwidwa ndi anti-chicken IgY antibody yomwe idayikidwa pa C line, ndipo mzere wa fulorosenti umapangidwa.Mzere wowongolera (C mzere) umagwiritsidwa ntchito ngati njira yoyendetsera.
Zolemba:
| Kupanga | Ndalama | Kufotokozera |
| IFU | 1 | / |
| Kaseti yoyesera | 20 | Chikwama chilichonse chosindikizidwa chokhala ndi chipangizo chimodzi choyesera ndi desiccant imodzi |
| sampuli diluent | 3mL*1 botolo | 20mM PBS, Sodium Casein, ProClin 300 |
| Micropipette | 20 | Micropipette yokhala ndi mzere wa 20μL |
| Lancet | 20 | / |
| Pepala la mowa | 20 | / |
Njira Yoyesera:
● Kutolere Magazi Kwachala
● Werengani zotsatira zake ndi chowunikira cha fluorescence
