Kuyesa kwa Rotavirus / Adenovirus / Norovirus Ag
Cholinga Chogwiritsidwa Ntchito
Zidazi zimapangidwira kuti zizindikire mwachindunji komanso moyenera ma antigen a gulu A a rotavirus, ma antigen adenovirus 40 ndi 41, norovirus (GI) ndi norovirus (GII) ma antigen mu ndowe za anthu.
Zotsatira zoyezetsa zabwino zimafuna kutsimikiziridwa kwina.Zotsatira zoyezetsa sizimachotsa kuthekera kwa matenda.
Zotsatira za mayeso a zidazi ndizongowona zachipatala zokha.Ndi bwino kuchita kusanthula mwatsatanetsatane za chikhalidwe zochokera wodwalayo matenda mawonetseredwe ndi zina zasayansi mayesero.
Mwachidule
Rotavirus (RV)ndi kachilombo kofunikira kamene kamayambitsa matenda otsekula m'mimba komanso matenda otsegula m'mimba mwa makanda ndi ana aang'ono padziko lonse lapansi.Chiwopsezo chachikulu cha matendawa ndi autumn, chomwe chimatchedwanso "kutsekula m'mimba kwa makanda ndi ana aang'ono".Chiwopsezo cha matenda obwera chifukwa cha ma virus kwa makanda mkati mwa miyezi ndi zaka 2 ndi okwera mpaka 62%, ndipo nthawi yobereketsa ndi masiku 1 mpaka 7, nthawi zambiri osakwana maola 48, kuwonetseredwa ndi kutsekula m'mimba kwambiri komanso kutaya madzi m'thupi.Ikalowa m'thupi la munthu, imabwerezanso m'maselo oyipa a epithelial a m'matumbo aang'ono ndipo imatulutsidwa mochuluka ndi ndowe.
Adenovirus (ADV)ndi kachilombo ka DNA kolowera pawiri komwe kumakhala 70-90nm.Ndi kachilombo ka symmetric icosahedral wopanda envelopu.Tinthu tating'onoting'ono ta virus timapangidwa makamaka ndi zipolopolo zamapuloteni komanso ma DNA omwe ali ndi zingwe ziwiri.Enteric adenovirus mtundu 40 ndi mtundu 41 wa kagulu F ndi tizilombo toyambitsa matenda otsekula m'mimba mwa anthu, makamaka makanda ndi ana aang'ono (osakwana zaka 4).Kutalika kwa makulitsidwe ndi masiku 3 mpaka 10.Imabwerezedwa m'maselo am'mimba ndipo imatuluka mu ndowe kwa masiku 10.chipatala mawonetseredwe ululu m`mimba, kutsegula m`mimba, madzi ndowe, limodzi ndi malungo ndi kusanza.
Norovirus (NoV)a m'banja la caliciviridae ndipo ali ndi tinthu ting'onoting'ono ta 20-hedral ndi m'mimba mwake 27-35 nm ndipo mulibe envelopu.Norovirus ndi imodzi mwa tizilombo toyambitsa matenda tomwe timayambitsa matenda osagwiritsa ntchito bakiteriya pakali pano.Kachilombo kameneka ndi kachilombo koyambitsa matenda ndipo kamafala makamaka ndi madzi oipitsidwa, chakudya, kukhudzana ndi kufalikira kwa mpweya wopangidwa ndi zowononga.Norovirus ndiye kachilombo kachiwiri kamene kamayambitsa matenda otsekula m'mimba mwa ana, ndipo imayamba m'malo odzaza anthu.Ma Noroviruses amagawika m'magulu asanu (GI, GII, GIII, GIV ndi GV), ndipo matenda akuluakulu omwe amapatsira anthu ndi GI, GII ndi GIV, pomwe ma genome a GII ndi omwe amapezeka kwambiri padziko lonse lapansi.Njira zodziwira zachipatala kapena za labotale za matenda a norovirus makamaka zimaphatikiza ma electron microscopy, biology ya mamolekyulu ndi kuzindikira kwa immunological.
Kupanga
Kusonkhanitsa ndi Kusamalira Zitsanzo
1. Sonkhanitsani ndowe zachisawawa m'chotengera chaukhondo, chowuma.
2. Tsegulani chipangizo chosonkhanitsira ndowe pomasula pamwamba ndikugwiritsa ntchito fosholo yosonkhanitsira mwachisawawa
3. kuboolani ndowe zachinyezi m'malo 2 ~ 5 osiyanasiyana kuti mutenge ndowe zolimba za 100mg (zofanana ndi 1/2 ya nandolo) kapena ndowe zamadzi 100μL.Osatenga ndowe chifukwa izi zitha kubweretsa zotsatira zolakwika.
4. Onetsetsani kuti ndowe zachitsanzo zili m'mizere ya fosholo yosonkhanitsira.Kuchulukira kwa ndowe kumatha kubweretsa zotsatira zosavomerezeka.
5. Yatsani ndi kumangitsa kapu pa chipangizo chosonkhanitsira zitsanzo.
6. Gwirani mwamphamvu chipangizo chosonkhanitsira ndowe.
Njira Yoyesera
1. Bweretsani chitsanzo ndi zigawo zoyesera ku firiji ngati zili mufiriji kapena zachisanu.
2. Mukakonzeka kuyesa, tsegulani thumba lomatapo ndikung'amba m'mphepete mwake.Chotsani mayeso m'thumba.
3. Ikani chipangizo choyesera pamalo oyera, ophwanyika.
4. Ikani chipangizo chotolera ndowe mowongoka ndi kupotoza kapu ya dispenser.
5. Kugwira chipangizo chosonkhanitsira ndowe molunjika, ikani 80μL (mozungulira madontho awiri) a yankho mu chitsime cha chitsanzo cha chipangizo choyesera.Osadzaza chitsanzo.
6. Werengani zotsatira za mayeso mkati mwa mphindi khumi ndi zisanu.Osawerenga zotsatira pambuyo pa mphindi 15.
Kutanthauzira kwa Zotsatira
1. Zabwino:Kukhalapo kwa mizere iwiri yofiirira (T ndi C) mkati mwazenera lazotsatira kukuwonetsa zabwino za antigen ya RV/ADV/NoV.
2. Zoipa:Mzere umodzi wofiyira-wofiirira womwe ukuwonekera pamzere wowongolera (C) ukuwonetsa zotsatira zoyipa.
3. Zosavomerezeka:Ngati mzere wowongolera (C) ukulephera kuwonekera, mosasamala kanthu kuti mzere wa T ukuwoneka kapena ayi, mayesowo ndi osavomerezeka.Unikaninso ndondomekoyi ndikubwereza kuyesanso ndi chipangizo chatsopano choyesera.
