Zogulitsa

Tsatanetsatane wa Zamalonda:

Innovita® 2019-nCoV IgM/IgG Test idapangidwa kuti izindikire komanso kuyang'anira miliri ya matenda omwe amayamba chifukwa cha buku la coronavirus (2019-nCoV).ORF1ab ndi N jini ya 2019-nCoV imadziwika bwino kuchokera ku nsonga zapakhosi ndi zitsanzo zamadzimadzi a alveolar lavage zomwe zimasonkhanitsidwa kuchokera ku milandu yomwe akuganiziridwa kuti ndi chibayo, odwala omwe akuganiziridwa kuti ndi omwe akuyenera kuwazindikira.
Zotsatira za mayeso a zidazi ndizongowona zachipatala zokha.Ndi bwino kuchita kusanthula mwatsatanetsatane za chikhalidwe zochokera wodwalayo matenda mawonetseredwe ndi zina zasayansi mayesero.

Mfundo Yofunika:

Chidachi chimagwiritsa ntchito sitepe imodzi reverse transcript polymerase chain reaction (RT-PCR) ukadaulo wozindikira kuti ugwirizane ndi jini ya coronavirus (2019-nCoV) ORF1ab, jini ya N, ndi mndandanda wamtundu wamtundu wamunthu.Zoyambira zapadera ndi ma probe a taqman adapangidwa m'magawo otetezedwa.

Zolemba:

Zindikirani: 1. Magulu osiyanasiyana a reagents sayenera kusakanikirana.
2. Kuwongolera kwabwino ndi zowongolera zabwino siziyenera kuchotsedwa

Njira Yoyesera:

1. Kutulutsa kwa nucleic acid:
Zida zochotsera zamalonda za RNA zilipo, kuchotsa mikanda ya maginito ndi kutulutsa kozungulira kumalimbikitsidwa pazida izi.
2. Konzani Zosakaniza Zosakaniza:

● Chotsani 2019-nCoV reaction Mix A/B ndi kusunga kutentha mpaka kusazizira;
● Tengani magawo ofanana (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) ndi kusakaniza, ndiyeno aliquot aliyense PCR anachita ndi 20μL/ chubu;
● Onjezani 5μL ya template ya RNA kapena kuwongolera koyipa kapena kuwongolera koyenera, kenako kuphimba chipewa cha chubu;
● Ikani chubu chochitira mu chipangizo cha fluorescence PCR, ndikukhazikitsa zowongolera / zabwino ndi magawo a chitsanzo cha RT-PCR molingana ndi malangizo ogwiritsira ntchito chida.
● Lembani Zitsanzo za kuyika kwadongosolo

3.RT-PCR protocol:

Zokonda Kovomerezeka: